تأثیر یک دوره تمرین تناوبی خیلی شدید بر کلاژن – 1 و TGF-β1 و عملکرد قلبی عضله قلب موش صحرایی پس از ایسکمی- ریپرفیوژن

Objective: This study was conducted to evaluate the effect of a period of high-intensity interval training (HIIT) on myocardial collagen-1 and TGF-&beta;1 and cardiac function in post ischemia-reperfusion rats. Materials and Methods: Thirty two (32) rats were anaesthetized by thiopental sodium. Intubation and connecting to ventilator and LAD ligation after thoracotomy was conducted. The ischemia was by 30 minutes and reperfusion was performed after ischemia. Rats were randomly divided into four groups: control-ischemia, Ischemia-HIIT, HIIT, and sham (HIIT groups without surgery, Sham groups without training). After 4 weeks of recovery, exercise training was performed (8 weeks, 3 days per week) 40 min including 5 min warm- up and cool- down and 30 min interval running at 85-90 % VO2max and separated by interval recovery at 50-60 % VO2max. 48 hours after last training session, rats were anesthetized and heart was removed. The gene expression of collagen-I and TGF-&beta;1 was measured with PCR-RT method. Results: Data analyzed showed that collagen 1 expression was significantly lower in Ischemia-HIIT group compared with control-ischemia group (p<0.05). Expression of TGF-&beta;1 was significantly lower in three groups as compared to control-ischemia group (p<0.05). In addition, EF (p=0.021) and FS (P=0.011) was significantly greater in Ischemia&ndash;HIIT group as compared to Control-ischemia. Conclusion: HIIT is an efficiency factor for decreasing TGF-&beta;1 and collagen-1 and increasing cardiac function.